欢迎浏览上海佐诚生物科技有限公司官网
登录|注册|关注官方微信
021-34060806

染色技术

您所在位置: 首页> 技术支持> 染色技术

HE染色

发表日期:2016年03月11日 点击击数: 1386

     染色程序
1.石蜡切片HE染色(常规HE染色)
(1)二甲苯Ⅰ                        5~10min
(2)二甲苯Ⅱ                       5~10min
(3)无水乙醇Ⅰ                     1~3min
(4)无水乙醇Ⅱ                    1~3min
(5)95%乙醇Ⅰ                     1~3min
(6)95%乙醇Ⅱ                    1~3min
(7)80%乙醇                      1min
(8)蒸馏水                         1min
(9)苏木素液染色                5~10min
(10)流水洗去苏木素液        1min
(11)1%盐酸-乙醇              1~3s
(12)稍水洗                       1~2s  
(13)返蓝(用温水或1%氨水等) 5~10s  
(14)流水冲洗                    1~2min
(15)蒸馏水洗                    1~2min
(16)0.5%伊红液染色          1~3min
(17)蒸馏水稍洗                 1~2s
(18)80%乙醇                    1~2s
(19)95%乙醇Ⅰ                   2~3min
(20)95%乙醇Ⅱ                  2~3min
(21)无水乙醇Ⅰ                   3~5min
(22)无水乙醇Ⅱ                  3~5min
(23)石炭酸-二甲苯             3~5min
(24)二甲苯Ⅰ                      3~5min
(25)二甲苯Ⅱ                     3~5min
(26)二甲苯Ⅲ                    3~5min
(27)中性树胶封固